Fig. 5

Overexpression of C3G in MKs enhances the survival of female mice following weekly 5-FU administration (A) Scheme illustrating the 5-FU administration schedule for the 5-FU challenge. Kaplan-Meyer curves depict the overall survival of mice after repeated weekly administration of 150 mg/kg 5-FU in (B–D) wtC3G^Pf4 and tgC3G^Pf4 mice, and (E–G) C3G^Pf4-wt and C3G^Pf4-KO mice. Results are shown for (B, E) all the mice tested, (C, F) only females and (D, G) only males. The first injection was performed on day 0. Each additional dose is represented as a dotted line in the plot. The difference in median survival and p-value according to the Log-rank (Mantel-Cox) test are shown in the squared box. Five to six independent experiments were performed. wtC3G^Pf4, n = 11 (4 females, 7 males); tgC3G^Pf4, n = 14 (5 females, 9 males); C3G^Pf4-wt, n = 17 (10 females, 7 males); C3G^Pf4-KO, n = 19 (6 females, 13 males). (H–K) Bar plots show the mean ± SEM of the log₂(Fold change) of mRNA expression analyzed by RT-qPCR of (H) Tpo, (I) Pf4/Cxcl4, (J) Sdf1/Cxcl12, and (K) Tgfb1 in CD41⁺CD42⁺ MKs isolated from the BM of tgC3G^Pf4 and C3G^Pf4-KO mice, and their corresponding wild-types. Values are relative to β-actin levels and normalized to the corresponding wild-type control. *p ≤ 0.05