Fig. 4

Sorcin decreased susceptibility to ferroptosis. (A-D) Representative images and statistical analysis of lipid ROS levels in shRNA-induced sorcin-knockdown cells after treatment with RSL3 or erastin for 48 h. (E-H) Representative images and statistical analysis of lipid ROS levels in sorcin-overexpressing cells after treatment with RSL3 or erastin for 48 h. (I) Analysis of the viability of shRNA-induced sorcin-knockdown cells after treatment with RSL3 or erastin for 36 h. (J) Analysis of the viability of sorcin-overexpressing cells after treatment with RSL3 or erastin for 48 h. (K) Statistical analysis of the migration of the indicated PANC-1 and Capan-2 cells after treatment with RSL3 (0.5 µM), erastin (1 µM), Ferro (5 µM), or Lipro (2 µM) for 48 h. (L) Representative images of the migration of the indicated Capan-2 cells. (M) Statistical analysis of the invasion of the indicated PANC-1 and Capan-2 cells after treatment with RSL3 (0.5 µM), erastin (1 µM), Ferro (5 µM), or Lipro (2 µM) for 48 h. (N) Representative images of cell invasion of the indicated Capan-2 cells