Fig. 2

Sorcin promoted pancreatic cancer progression. (A) The expression level of sorcin in pancreatic cancer cells. (B) Western blotting analysis of sorcin and ALDH1A1 expression in PANC-1, AsPC-1, Capan-2, PaTu8988T and MIA Paca-2 pancreatic cancer cells after sorcin was knocked down by shRNA or overexpressed. (C) Representative images of the migration and invasion of PANC-1, Capan-2 and AsPC-1 cells after sorcin was knocked down by shRNA or overexpressed. (D-E) Statistical analysis of the migration and invasion of PANC-1, Capan-2 and AsPC-1 cells after sorcin was knocked down by shRNA or overexpressed. (F) Representative images and statistical analysis of the tumorsphere formation rate after sorcin knockdown or overexpression. (G-H) Representative images and statistical analysis of ALDH⁺ PANC-1 cells after sorcin knockdown. (I-J) Representative images and statistical analysis of ALDH⁺ Capan-2 cells after sorcin knockdown. (K-L) Images and growth rates of tumors from mice engrafted with sorcin-knockdown PANC-1 cells, n = 5. (M) Representative images of immunohistochemical staining and statistical analysis of sorcin and ALDH1A1 expression levels in xenograft tumor tissues