Fig. 4

RT plus YM101 attenuates tissue fibrosis and increases infiltration. (a-f) 4T1 tumor-bearing mice received isotype, YM101, RT or combination therapy. Tumor tissues were harvested at day 10 post RT. Representative IHC images and quantifications of tumor sections stained with an anti-α-SMA, anti-collagen I and anti-CD8 antibody. Scale bars represent 50 and 100 μm. Each point on the graphs represents an individual fields (N = 15 fields, 5 mice). (g) The representative CD4 (green) and CD8 (red) IF staining of tumor periphery and center. Dashed line indicates tumor boundaries. Scale bars represent 50 μm. (h) Quantification of infiltration depth of T cells based on IHC. The P values were determined using one-way ANOVA followed by Tukey’s multiple comparisons test. P < 0.01; * P < 0.001; **** P < 0.0001; ns: not significant