Fig. 4

Ipilimumab is associated with increased early NK cell proliferation but more rapid contraction of the NK cell compartment. A Distribution of NK cells and T cells in the peripheral blood as a proportion of total lymphocytes, stratified by IPI treatment, as evaluated with flow cytometry. The data presented is a percentage of total lymphocytes. B Distribution of peripheral blood mononuclear cells as measured with single cell CITE-seq on day + 7 (D7) and day + 28 (D28), comparing IPI treated (IPI⁺, n = 2) and IPI untreated (IPI⁻, n = 2) patients. C UMAP of NK cell clusters defined with single cell CITE-seq, separated by time points assessed and IPI treatment. The color scheme corresponds to the same populations as shown in B. D Gene set enrichment analysis of pathways in NK cell clusters comparing IPI⁺ (n = 2) and IPI⁻ (n = 2) patients on day + 7 (D7)