Fig. 2

GD induces purine nucleotide degradation. A Metabolite set enrichment of the polar metabolites from HCT116 cells with GD versus normal culture as determined by untargeted metabolomics analysis (XCMS). B Metabolite set enrichment of the polar metabolites from DLD1 cells with GD versus normal culture as determined by untargeted metabolomics analysis (XCMS). C Metabolic pathway enrichment analysis of changed metabolites from HCT116 cells under GD determined by targeted metabolomics analysis (quantitative 600MRM analysis). Purine metabolism was marked with a yellow box. D Levels of metabolites involved in adenine nucleotide degradation from HCT116 cells under normal conditions or GD (0 mM, 4 h). E Levels of metabolites involved in guanine nucleotide degradation from HCT116 cells under normal conditions or GD (0 mM, 4 h). F Pie chart of nucleotide associated metabolic pathways among GD-induced changed metabolic pathways in YAP activated or YAP non-activated cells. In (D), (E), data are the mean ± S.D.; P values were calculated using a two-tailed unpaired Student’s t-test. *, P < 0.05; **, P < 0.01; ns, no significance