Fig. 2

In vitro anti-tumor activity of CAR.GD2 T-cells correlates to GD2 expression on human sarcoma cell lines. (A, top panel) Expression of GD2 in human osteosarcoma (OS) cell lines (143B, MG-63, U-2OS, HOS and SAOS-2); (B, top panel) in embryonal rhabdomyosarcoma (ERMS) cell lines (RD, CT10); (C, top panel) in ARMS cell lines (SCMC, RH4, RH41 and RH30); (D, top panel) in Ewing Sarcoma cell lines (SK-ES-1, A-673) as assessed by flow cytometry. The Isotype control was run for each tumor cell line (white). Five-day co-cultures were performed in 6 to 16 independent experiments, in which GFP + OS (A, bottom panel), ERMS (B, bottom panel) and ARMS (C, bottom panel), Ewing (D, bottom panel) cell lines were co-cultured with NT T or CAR.GD2 T-cells at the effector-target (E: T) ratio of 1:1. Paired t-Test was used as comparison test. *p < 0.05, **p < 0.01, ****p-value = < 0.0001