Fig. 1

GD2-synthase (B4GALNT1) mRNA gene expression profile in solid tumours. A Box plot depicting B4GALNT1 expression across cancers and healthy tissues. Data of TGCA and GTEX datasets showed expression level of B4GALNT1 in both normal tissues (green) and tumor patients with solid tumours (red). B B4GALNT1 expression on paediatric sarcoma cancer (Oncogenomics dataset—probe 206435_at) compared to Healthy Tissue (HT) control. C Kaplan–Meier analysis for overall survival in OS (TARGET dataset), RMS (ITCC dataset) and for Ewing’s sarcoma (EWS) (Dirksen dataset) for B4GALNT1 mRNA expression. The percentage of GD2 positive cells D and GD2 MFI E on paediatric sarcoma tissues, evaluated by FACS analysis, compared to PBMC negative control. Two-way Anova was used as multi comparison test. *p < 0.05, **p < 0.01, ***p < 0.001, ****p-value = < 0.0001