Fig. 7
From: Remodeling of anti-tumor immunity with antibodies targeting a p53 mutant
The anti-tumor effect induced by E285K-mAb in the IgG1 format requires TRIM21. A Protein interaction between TRIM21 and E285K-mAb assayed by FACS in MC38-p53KO/E285K cell treated with E285K-mAb and TRIM21-mAb. B and C Detection of TRIM21 knockdown efficiency and p53-E285K expression levels post siRNA treatment in MC38-p53KO/E285K cells by western blot. D and E Analysis of CD103+CD11c+ and CD8+CD11c+ cell percentages in tumors from mice treated with LNP-TRIM21 siRNA and LNP-pE285K-mAb (n = 5 mice). F and G The expression of activation markers (CD80, CD86, and MHC) on CD11c+ cells within tumors using FACS. H and I IL-2, IFN-γ, and TNF-α expression on CD8+ T cells. J Tumor volumes measured at different times after inoculation. K Tumor diameter of individual mice from the groups in (j) as a function of time. Each group consisted of five tested mice. L Animal survival (n = 10 mice). Data presented as means ± SD. Statistical significance was set at **p < 0.01, ***p < 0.001, and ****p < 0.0001