Figure 3

A comparison of in vitro activity between G-CSFa and wild-type G-CSF. Recombinant G-CSFa and G-CSF at indicated concentrations (10 pg, 20 pg, 100 pg, 200 pg, 1 μg, and 20 μg per milliliter, respectively) were used for the stimulation of NFS-60 cell proliferation. The concentrations that stimulate 50% cell proliferation rate (ED50) were obtained for each cytokine. Each experiment was repeated for at least three times and similar results were obtained.