Figure 6

Increasing volume of CD16 beads resulted in HER2 signal depletion. Negative selection was performed on 3 PBL samples per 3 normal subjects with increasing amounts of CD16-labelled immunomagnetic beads. HER2 RNA expression was measured in both the CD16 selected cells (beads) and unselected cells (supernatant) by Real-time qRT-PCR. Data is expressed as the percent HER2 signal in each subfraction (bead or supernatant) over total HER2 signal for that subject.